Detection of Malaria Parasite Protein in Urine of Patients with Acute Uncomplicated Malaria Using Rapid Diagnostic Test Kits

Objectives: The invasive nature of the current malaria diagnostic techniques impairs compliance to diagnosis, especially for on-field detection. Adapting non-invasive methods biological sample collection rapid diagnosis infections may provide a more efficient approach case management and epidemiological studies malaria. This study was designed evaluate detection Plasmodium falciparum Histidine-rich Protein II (PfHRP-2) in urine samples optimization as markers P. infection. 
 Methods: One hundred (100) microscopically confirmed patients with infection 25 negative controls were recruited study. Blood all participants tested presence PfHRP-2 using Rapid Diagnostic Test (RDT) kits. In addition, malaria-infected analyzed CareStartTM Global Devices (USA) Malaria performances RDT kits evaluated.
 Results: Overall, two brands diagnostics demonstrated 71% sensitivity (95%CI=62.1-79.9%) 96% specificity (95%CI=88.3-103.7%) urine. sensitivities tests at asexual parasitemia ? 2000 ?L-1 > 69.6% (95%CI=56.3-82.9%) 72.2% (95%CI=60.3-84.2%) respectively. had individual 80% (95%CI= 65.7-94.3%) 67.1% 56.1-78.1%) respectively (P= 0.072).
 Conclusion: Findings revealed that urine-based RDTs have limited capacities due their low require optimizations meet required standards. J Microbiol Infect Dis 2022; 12(3):97-107.